HIF-1 alpha, Hydroxy Pro564 Antibody, Novus Biologicals™

Catalog No.	Quantity
NB11074679	0.1 mL

Catalog No. NB11074679 Supplier Novus Biologicals Supplier No. NB11074679

Description

Rabbit Polyclonal Antibody has been used in 6 publications

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoblotting.

Specifications

• Antigen: HIF-1 alpha (Hydroxy Pro564)
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoblot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blot 1:500-1:2000, ELISA 1:5000-1:20000, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100-1:2000
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Accession No.: Q16665
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Host Species: Rabbit
• Immunogen: This HIF-1 alpha [Hydroxy Pro564] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha. (Uniprot: Q16665)
• Molecular Weight of Antigen: 93 kDa
• Purification Method: Unpurified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus species sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Target Species: Human, Mouse, Rat, Bovine, Canine, Monkey, Xenopus species
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
• Form: Antisera

For Research Use Only