HIF-1 alpha, Hydroxy Pro564 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 6 publications

Supplier:  Novus Biologicals NB11074679

Catalog No. NB11074679

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoblotting.

Specifications

• Antigen: HIF-1 alpha (Hydroxy Pro564)
• Classification: Polyclonal
• Dilution: Western Blot 1:500-1:2000, ELISA 1:5000-1:20000, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100-1:2000
• Gene Accession No.: Q16665
• Gene Symbols: HIF1A
• Immunogen: This HIF-1 alpha [Hydroxy Pro564] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha. (Uniprot: Q16665)
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus species sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoblot
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Host Species: Rabbit
• Molecular Weight of Antigen: 93 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Bovine, Canine, Monkey, Xenopus species
• Form: Antisera

For Research Use Only