HIF-2 alpha/EPAS1 Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 663 publications

Supplier:  Novus Biologicals NB100122

Catalog No. NB100122

Description

HIF-2 alpha/EPAS1 Polyclonal specifically detects HIF-2 alpha/EPAS1 in Human, Mouse, Rat, Fish, Hamster, Primate, Rabbit, Reptile, Sheep samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, In vitro assay, Gel Super Shift Assays, SDS-Page, Knockout Validated, Knockdown Validated, Dual RNAscope ISH-IHC.

Specifications

• Antigen: HIF-2 alpha/EPAS1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% Sodium Azide
• Gene Alias: anti hif 2 alpha, anti-HIF-2 alpha, Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain protein 1, endothelial PAS domain-containing protein 1, EPAS1, EPAS-1, HIF-1-alpha-like factor,
• Host Species: Rabbit
• Molecular Weight of Antigen: 96.5 kDa
• Quantity: 0.1 mL
• Research Discipline: Angiogenesis, Apoptosis, Cancer, Cancer Stem Cells, Chromatin Research, Embryonic Stem Cell Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Transcription Factors and Regulators
• Gene ID (Entrez): 2034
• Target Species: Human, Mouse, Rat, Fish, Hamster, Primate, Rabbit, Reptile, Sheep
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1 - 2 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 5 ug/ 1 mg lysate, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28115701), In vitro assay reported in scientific literature (PMID 24998849), Gel Super Shift Assays reported in scientific literature (PMID 15184875), SDS-Page, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated reported in scientific literature (PMID 26861754), KnockDown Validated reported in scientific literature (PMID 31061092), Dual RNAscope ISH-IHC
• Gene Accession No.: Q99814
• Gene Symbols: EPAS1
• Immunogen: This HIF-2 alpha/EPAS1 Antibody was developed against a peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This HIF-2 alpha/EPAS1 Antibody is specific for HIF-2 alpha/EPAS, and does not cross-react with HIF-1 alpha.
• Content And Storage: Store at -20 degreesC.

For Research Use Only