HIF-2 alpha/EPAS1 Antibody (ep190b), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 177 publications

Supplier:  Novus Biologicals NB100132

Catalog No. NB100132

Description

HIF-2 alpha/EPAS1 Monoclonal specifically detects HIF-2 alpha/EPAS1 in Human, Mouse, Rat, Bovine, Hamster samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vivo assay, Gel Supershift Assay, Knockdown Validated.

Specifications

• Antigen: HIF-2 alpha/EPAS1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1 - 2 ug/mL, Simple Western 1:100, Flow Cytometry 1:400, ELISA 1:100-1:2000, Immunohistochemistry 1:150 - 1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:150 - 1:300, Immunohistochemistry-Frozen reported in scientific literature (PMID 24973414), Gel Super Shift Assays reported in scientific literature (PMID 17404621), In vivo assay reported in scientific literature (PMID 23857308), Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP), KnockDown Validated reported in scientific literature (PMID 32054096)
• Gene Alias: Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain protein 1, endothelial PAS domain-containing protein 1, ep190b, EPAS1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-
• Host Species: Mouse
• Molecular Weight of Antigen: 96.5 kDa
• Quantity: 0.1 mL
• Research Discipline: Angiogenesis, Apoptosis, Cancer, Cancer Stem Cells, Chromatin Research, Embryonic Stem Cell Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Transcription Factors and Regulators
• Gene ID (Entrez): 2034
• Target Species: Human, Mouse, Rat, Bovine, Hamster
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown
• Clone: ep190b
• Conjugate: Unconjugated
• Gene Accession No.: Q99814
• Gene Symbols: EPAS1
• Immunogen: The immunogen recognized by this HIF-2 alpha/EPAS1 Antibody (ep190b) maps to a region between amino acids 535-631. [UniProt# Q99814]
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This HIF-2 alpha/EPAS1 Antibody (ep190b) is specific for HIF-2 alpha/EPAS1, and does not cross-react with HIF-1 alpha.
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Isotype: IgG1

For Research Use Only