HIF1-alpha Recombinant Rabbit Monoclonal Antibody (BL-124-3F7), Bethyl Laboratories

Rabbit Recombit Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-157-1258	Bethyl Laboratories, Inc A700001T	20 μL
50-157-1257	Bethyl Laboratories, Inc A700001	100 μL

Description

The recommended shelf life for this product is 1 year from date of receipt.

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Specifications

• Antigen: HIF1-alpha
• Applications: ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: Between 433 and 826
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: HIF1A

• Clone: BL-124-3F7
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
• Gene Alias: Hypoxia inducing factor 1
• Gene Symbols: HIF1A
• Isotype: IgG
• Purification Method: Ion-exchange chromatography
• Product Type: Antibody