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Histone H2Av, p Ser137 Antibody, Novus Biologicals™
SDP

Catalog No. NBP178103 Shop All R&D Systems Products
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Rabbit Polyclonal Antibody

Histone H2Av Polyclonal specifically detects Histone H2Av in Drosophila samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

Specifications

Antigen Histone H2Av (p Ser137)
Applications Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration 1.1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:400-1:1600, ELISA 1:29000-1:30000, Immunohistochemistry 2 ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P08985
Gene Alias CG5499 gene product from transcript CG5499-RA, gamma-His2Av, H2AX, 5499, CG5499, CG5499-PA, Dmel\CG5499, gamma-H2Av, gamma-HIS2AV, H2A.F/Z, H2a.V, H2A.Z, H2av, H2AV_DROM, h2AvD, H2AZ, his, His2, HIS2AV, His2AvD, HisH2Av, Hist, Hist2av, Histone H2A variant, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602
Gene Symbols His2Av
Host Species Rabbit
Immunogen Histone H2Av [p Ser137] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein. (Uniprot: P08985)
Molecular Weight of Antigen 14.98 kDa
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 43229
Test Specificity Affinity purified Anti-Histone Histone H2Av pS137 Antibody is directed against the phosphorylated form of Drosophila Histone H2Av protein at the pS137 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila Histone H2Av pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila Histone H2Av is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with Histone H2Av proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Species Drosophila
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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