Histone H2Av, p Ser137 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP178103

Catalog No. NBP178103

Description

Histone H2Av Polyclonal specifically detects Histone H2Av in Drosophila samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: Histone H2Av (p Ser137)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: CG5499 gene product from transcript CG5499-RA, gamma-His2Av, H2AX, 5499, CG5499, CG5499-PA, Dmel\CG5499, gamma-H2Av, gamma-HIS2AV, H2A.F/Z, H2a.V, H2A.Z, H2av, H2AV_DROM, h2AvD, H2AZ, his, His2, HIS2AV, His2AvD, HisH2Av, Hist, Hist2av, Histone H2A variant, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602
• Host Species: Rabbit
• Molecular Weight of Antigen: 14.98 kDa
• Quantity: 0.1 mg
• Research Discipline: Phospho Specific
• Gene ID (Entrez): 43229
• Target Species: Drosophila
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Concentration: 1.1 mg/ml
• Dilution: Western Blot 1:400-1:1600, ELISA 1:29000-1:30000, Immunohistochemistry 2 ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin
• Gene Accession No.: P08985
• Gene Symbols: His2Av
• Immunogen: Histone H2Av [p Ser137] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein. (Uniprot: P08985)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Affinity purified Anti-Histone Histone H2Av pS137 Antibody is directed against the phosphorylated form of Drosophila Histone H2Av protein at the pS137 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila Histone H2Av pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila Histone H2Av is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with Histone H2Av proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only