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Histone H2AX, p Ser139 Antibody (3F2), Novus Biologicals™
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Catalog No. NB10074435 Shop All R&D Systems Products
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NB10074435 100 μg
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Catalog No. NB10074435 Supplier Novus Biologicals Supplier No. NB10074435
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Description

Mouse Monoclonal Antibody has been used in 6 publications

Histone H2AX (p Ser139) Monoclonal specifically detects Histone H2AX (p Ser139) in Bovine, Human, Mouse, Rabbit samples. It is validated for ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Simple Western, Western Blot.

Specifications

Antigen Histone H2AX (p Ser139)
Applications ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Simple Western, Western Blot
Classification Monoclonal
Clone 3F2
Concentration 1 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1 ug/ml, Simple Western 10 ug/ml, Flow Cytometry 1 ug 10^6 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 ug/ml, Immunohistochemistry-Paraffin 1:10 - 1:500
Gene Accession No. P16104
Gene Alias H2A.X, H2A/X, H2AFX
Gene Symbols H2AFX
Host Species Mouse
Immunogen This Histone H2AX [p Ser139] Antibody (3F2) was developed against a synthetic peptide sequence surrounding phosphorylated Ser139.
Molecular Weight of Antigen 15 kDa
Purification Method Protein G purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Mitotic Regulators, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 3014
Test Specificity In Western blot this antibody detects ∼17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed. Used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section.
Target Species Bovine, Human, Bovine, Mouse, Rabbit
Content And Storage Store at -20C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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