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Histone H3, p Ser28 Antibody (HTA28), Novus Biologicals™
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Catalog No. NB6001168 Supplier Novus Biologicals Supplier No. NB6001168
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Description

Rat Monoclonal Antibody has been used in 8 publications

Histone H3 Monoclonal specifically detects Histone H3 in Human, Mouse, Bovine, Hamster samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray.

Specifications

Antigen Histone H3 (p Ser28)
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone HTA28
Concentration 0.5 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1.0 ug/ml, Flow Cytometry 1:10-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray
Gene Accession No. P84243
Gene Alias H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3S28p, HIST2H3C, histone H3.3, MGC87782, MGC87783
Gene Symbols H3C14
Host Species Rat
Immunogen This Histone H3 [p Ser28] antibody (HTA28) was raised against synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 (pSer28) of Human Histone H3.
Molecular Weight of Antigen 15 kDa
Purification Method Affinity Purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Loading Controls
Primary or Secondary Primary
Gene ID (Entrez) 126961
Test Specificity Histone H3 [p Ser28] antibody (HTA28) does not detect the unphosphorylated epitope. It detects the phosphorylated histone molecule at the onset of mitosis (prophase, metaphase and weaker at the beginning of anaphase), but not during late anaphase.
Target Species Human, Mouse, Bovine, Hamster
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype IgG2a
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For Research Use Only

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