Histone H3, Trimethyl Lys9 Antibody (6F12-H4), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 19 publications

Supplier:  Novus Biologicals NBP130141

Catalog No. NBP130141

Description

Histone H3 Monoclonal specifically detects Histone H3 in Human, Mouse, Rat, Porcine, C. elegans, Drosophila, Invertebrate, Mammal, Yeast samples. It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Histone H3 (Trimethyl Lys9)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot 1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:1000. Use reported in scientific literature (PMID 23872316), Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 28270554), Immunohistochemistry-Paraffin 1:1000, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
• Gene Alias: H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3K9Me3, HIST2H3C, histone H3.3, MGC87782, MGC87783
• Host Species: Mouse
• Molecular Weight of Antigen: 15 kDa
• Quantity: 0.1 mL
• Research Discipline: Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Loading Controls
• Gene ID (Entrez): 126961
• Target Species: Human, Mouse, Rat, Pig, C. elegans, Drosophila, Invertebrate, Mammalia, Yeast
• Form: Purified

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Clone: 6F12-H4
• Conjugate: Unconjugated
• Gene Accession No.: P84243, P84244
• Gene Symbols: H3C14
• Immunogen: This Histone H3 [Trimethyl Lys9] antibody (6F12-H4) was raised against a synthetic peptide made to an N-terminal region of Histone H3 (between amino acids 1-50). [UniProt# P84243]
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: All mammals including human, mouse and rat, Drosophila, C. elegans, yeast. Invertebrate/Blattella germanica (German cockroach) reactivity reported in scientific literature (PMID: 23872316).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only