HLA-A2 Monoclonal Antibody (BB7.2), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
12-987-642	Invitrogen™ 12987642	100 Tests

Description

Description: This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, which is a member of the HLA-A family. MHC class I exists as a single alpha chain composed of three domains. This molecule associates with beta 2-microglobulin and is expressed on all nucleated cells. The BB7.2 monoclonal antibody has been reported to exhibit blocking activity, as well as recognize HLA-A28. Applications Reported: This BB7.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BB7.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-A2
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P04439
• Gene Symbols: HLA-A
• Primary or Secondary: Primary
• Product Type: Antibody

• Clone: BB7.2
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3105
• Isotype: IgG2b κ
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: HLA-A