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HLA Class I Antibody (MEM-147) - BSA Free, Novus Biologicals™
SDP

Catalog No. NB500411 Shop All R&D Systems Products
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Catalog No. NB500411 Supplier Novus Biologicals Supplier No. NB500411
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Mouse Monoclonal Antibody

HLA Class I Monoclonal specifically detects HLA Class I in Human samples. It is validated for Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready.

Specifications

Antigen HLA Class I
Applications Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF
Classification Monoclonal
Clone MEM-147
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:1000, Flow Cytometry 1-5 ug/ml, Immunoprecipitation 1:50, CyTOF-ready
Gene Accession No. P04439
Gene Alias A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
Gene Symbols HLA-A
Host Species Mouse
Immunogen PHA-activated peripheral blood lymphocytes
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Adaptive Immunity, Cell Biology, Immunology
Primary or Secondary Primary
Gene ID (Entrez) 3105
Test Specificity This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Target Species Human
Content And Storage Store at 4C. Do not freeze.
Isotype IgG1
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