HLA Class I Antibody (MEM-147), Janelia Fluor™ 549, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB500411JF549

Catalog No. NB500411549

Description

HLA Class I Monoclonal specifically detects HLA Class I in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: HLAClassI
• Classification: Monoclonal
• Conjugate: Janelia Fluor 549
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: HLA-A
• Immunogen: PHA-activated peripheral blood lymphocytes
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: MEM-147
• Dilution: Flow Cytometry
• Gene Alias: A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
• Host Species: Mouse
• Purification Method: Protein A purified
• Research Discipline: Adaptive Immunity, Cell Biology, Immunology
• Gene ID (Entrez): 3105
• Target Species: Human
• Form: Purified