HLA-DR Antibody (LN-3), Alexa Fluor™ 488, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247670AF488

Catalog No. NB247670488

Description

HLA-DR Monoclonal specifically detects HLA-DR in Human, Monkey, Mouse (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: HLA-DR
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: HLA-DRA
• Immunogen: Activated human peripheral blood mononuclear cells
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG2b κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: LN-3
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
• Gene Alias: FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cell Biology, Cellular Markers, Diabetes Research, Immunology, Major Histocompatibility Complexes
• Gene ID (Entrez): 3122
• Target Species: Human, Monkey, Mouse (Negative)
• Form: Purified