Tocris Bioscience™ Hoechst Janelia Fluor 646

Description

Fluorogenic, red-emitting DNA probe

Hoechst Janelia Fluor™ 646 is a fluorogenic red-emitting DNA probe; it preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor™ 526 for multiplexing experiments. Hoechst Janelia Fluor™ 646 can also be combined with Hoechst Janelia Fluor™ 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor™ 646 in E. coli cells (∽100 nM - 1 μM).

Hoechst Janelia Fluor™ 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Hoechst Janelia Fluor™ 646 exhibits a quantum yield (QY) of 0.54; extinction coefficicent 152, 000; excitation maximum = 655 nm; emission maximum = 670 nm.

Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor™ 646 is suitable for multicolor microscopy experiments and for use in live cell imaging.

To measure the absorbance spectrum of Hoechst Janelia Fluo™ 646 we recommend the following solvent: TFE plus 0.1% TFA.

Specifications

• Chemical Name or Material: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1 H,3 'H-[2,5 '-bibenzo[d]imidazol]-2 '-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoat
• CAS: 2462011-60-3
• Quantity: 100 μg
• Target: Fluorescent DNA Stains
• Molecular Formula: C₆₄H₇₀N₁₀O₇Si
• Purity: 0.90