HSP27 Antibody (G3.1), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234621AF750

Catalog No. NB006249

Description

HSP27 Monoclonal specifically detects HSP27 in Human, Mouse, Rat, Chicken, Chimpanzee, Monkey, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: HSP27
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: HSPB1
• Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Phospho Specific
• Test Specificity: monoclonal antibody G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: G3.1
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Chicken, Primate, Sheep
• Isotype: IgG1 κ