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HSP27 Antibody (SPM252), Alexa Fluor™ 750, Novus Biologicals™
SDP

Catalog No. NB006532 Shop All R&D Systems Products
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NB006532 0.1 mL
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Catalog No. NB006532 Supplier Novus Biologicals Supplier No. NBP234751AF750
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Description

Mouse Monoclonal Antibody

HSP27 Monoclonal specifically detects HSP27 in Human, Mouse, Rat, Chicken, Chimpanzee, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen HSP27
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone SPM252
Conjugate Alexa Fluor 750
Dilution Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Formulation 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols HSPB1
Host Species Mouse
Immunogen Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Purification Method Protein A or G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Phospho Specific
Primary or Secondary Primary
Test Specificity It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Target Species Human, Mouse, Rat, Chicken, Primate, Sheep
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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