HSP27 Mouse, Clone: SPM252, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2328650.1MG

Catalog No. NBP232865A

Description

HSP27 Monoclonal specifically detects HSP27 in Human, Mouse, Rat, Chicken, Chimpanzee, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: HSP27
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
• Host Species: Mouse
• Molecular Weight of Antigen: 27 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Phospho Specific
• Gene ID (Entrez): 3315
• Target Species: Human, Mouse, Rat, Chicken, Primate, Sheep
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: SPM252
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
• Gene Accession No.: P04792
• Gene Symbols: HSPB1
• Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ