HSP90 alpha, ac Lys294 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP177944

Catalog No. NBP177944

Description

HSP90 alpha Polyclonal specifically detects HSP90 alpha in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: HSP90 alpha (ac Lys294)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38
• Host Species: Rabbit
• Molecular Weight of Antigen: 84.66 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Hypoxia, Membrane Trafficking and Chaperones
• Gene ID (Entrez): 3320
• Target Species: Human, Mouse, Rat, Chicken, Drosophila
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Concentration: 0.96 mg/ml
• Dilution: Western Blot 1:500-1:2500, ELISA 1:2500-1:10000, Immunohistochemistry 5-10 ug/ml, Immunohistochemistry-Paraffin 20 ug/ml
• Gene Accession No.: P46633, P07900, P07900, P07900, P07900
• Gene Symbols: HSP90AA1
• Immunogen: This affinity purified HSP90 alpha [ac Lys294] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human HSP90 alpha. (Uniprot: P07900)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This affinity purified antibody is directed against human HSP90 alpha protein acetylated at K294. While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with HSP90 alpha from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with HSP90 alpha from other species is unknown.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only