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Tango™ HTR2A-bla U2OS DA Assay Kit

Catalog No. K1613
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Catalog No. K1613 Supplier LIFE TECHNOLOGIES Supplier No. K1613
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Description

Tango™ HTR2A-bla U2OS DA Assay Kit contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site.

  • Gene Symbol: HTR2A
  • Accession Number: NM_000621.2
  • Cytoplasmic Tail: Tail from AVPR2 GPCR
  • Population Status: Single Cell Clone Origin
  • Includes: Tango™ HTR2A-bla U2OS DA cells, LiveBLAzer™-FRET B⁄G Loading Kit, 70 μg, Solution D, Tango™ HTR2A-bla U2OS DA Assay Protocol, LiveBLAzer™-FRET B⁄G Loading Protocol, Certificate of Analysis
  • The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
  • The Tango™ HTR2A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000621.2
Assay Entry Cell-based beta-arrestin recruitment
Cell State Division-Arrested Cells
Content And Storage K1613 Tango™ HTR2A-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• Tango™ HTR2A-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70μg
• Solution D
• Tango™ HTR2A-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ HTR2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Detection Method Fluorescence
Gene Symbol HTR2A
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Protein Family GPCR (G-Protein Coupled Receptors)
Quantity 1 Kit
Readout End Point
Reporter Gene BLA (Beta-Lactamase)
Target Entry HTR2A
Type Division-Arrested Cells Assay Kit
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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