Human B7-1/CD80 Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 9 publications

Supplier:  R&D Systems MAB140500

Catalog No. MAB140500

Description

B7-1/CD80 Monoclonal specifically detects B7-1/CD80 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA Capture (Matched Antibody Pair).

Specifications

• Antigen: B7-1/CD80
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 0.2-1 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
• Gene Accession No.: P33681
• Gene Symbols: CD80
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80 Val35-Asn242 (predicted) Accession # P33681
• Quantity: 500 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
• Target Species: Human
• Form: Supernatant

• Applications: Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF, In Situ Hybridization (ISH), ELISA
• Clone: 37711
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 941
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG1