Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-C_T™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:

• AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.

Specifications

• Concentration: 2X (Master Mix), 125X (RT Enzyme Mix)
• Content And Storage: Sufficient for 100 reactions at 20 μL reaction volume and includes:
  • 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.
  • 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.
  
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: SYBR
• Format: Tube
• PCR Method: 1-step RT-qPCR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Kit Type: Green RNA-to-CT 1-Step Kit
• Label or Dye: SYBR Green
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: RNA-to-C_T™, SYBR™
• Product Type: Real Time PCR SYBR Master Mix
• Purity or Quality Grade: UP (Ultra Pure)
• Quantity: 100 Reactions
• Reverse Transcriptase: ArrayScript™ UP
• Run Time: Standard
• Shipping Condition: Wet Ice
• Sufficient For: 100 Reactions at 20 μL
• For Use With (Application): Gene Expression
• No. of Reactions: 100 Reactions
• Sample Type: RNA
• Volume: 1 x 1 mL

For Research Use Only. Not for use in diagnostic procedures.