ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2443310.02MG

Catalog No. NBP24433100

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ICAM-1/CD54 Monoclonal specifically detects ICAM-1/CD54 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Functional, Immunofluorescence.

Specifications

• Antigen: ICAM-1/CD54
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: BB2, CD54, CD54 antigen, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor, P3.58
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
• Content And Storage: Store at 4C.
• Isotype: IgG2b κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: W-CAM-1 (same as Wehi-CAM-1 or 1H4)
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: ICAM1
• Immunogen: Raji Burkitt lymphoma cells
• Quantity: 0.02 mg
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Immunology, Mesenchymal Stem Cell Markers, Stem Cell Markers
• Gene ID (Entrez): 3383
• Target Species: Human
• Form: Purified

For Research Use Only