ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)), Novus Biologicals™

Catalog No.	Quantity
NBP24433101	0.1 mg
NBP24433100	0.02 mg
NBP24433102	0.2 mg

Catalog No. NBP24433101 Supplier Novus Biologicals Supplier No. NBP2443310.1MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ICAM-1/CD54 Monoclonal specifically detects ICAM-1/CD54 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Functional, Immunofluorescence.

Specifications

• Antigen: ICAM-1/CD54
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Classification: Monoclonal
• Clone: W-CAM-1 (same as Wehi-CAM-1 or 1H4)
• Concentration: 0.2mg/mL
• Conjugate: Unconjugated
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: BB2, CD54, CD54 antigen, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor, P3.58
• Gene Symbols: ICAM1
• Host Species: Mouse
• Immunogen: Raji Burkitt lymphoma cells
• Purification Method: Protein A or G purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Immunology, Mesenchymal Stem Cell Markers, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 3383
• Test Specificity: Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
• Target Species: Human
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only