Invitrogen™ IDH1 Polyclonal Antibody

Catalog No.	Quantity
PIPA528206	100 μL

Catalog No. PIPA528206 Supplier Invitrogen™ Supplier No. PA528206

Description

Rabbit Polyclonal Antibody

Recommended positive controls: A431, HepG2, HeLa, Huh-7, Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2, DDDDk-tagged IDH1-transfected 293T. Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Specifications

• Antigen: IDH1
• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 0.69 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: IDH1
• Gene Accession No.: O75874, O88844, P41562
• Gene Alias: AI314845; AI788952; cb876; corneal epithelial crystallin; cytosolic NADP+-dependent isocitrate dehydrogenase; cytosolic NADP-isocitrate dehydrogenase; cytosolic NADP-isocitrate dehydrongenase; E030024J03Rik; epididymis luminal protein 216; epididymis secretory protein Li 26; fm90e09; HEL-216; HEL-S-26; ICDH; Id-1; IDCD; IDH; Idh1; Idh-1; IDP; Idpc; im:7143416; isocitrate dehydrogenase (NAD(+)) IDH1; isocitrate dehydrogenase (NADP(+)) 1; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial; Isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase 1, soluble; Isocitric dehydrogenase; N2690; NAD(+)-specific ICDH; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP-cICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; OTTHUMP00000206464; OTTHUMP00000206465; OTTHUMP00000206466; oxalosuccinate decarboxylase; PICD; wu:fm90e09; YNL037C
• Gene Symbols: IDH1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15926, 24479, 3417
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG