IFN-alpha 2 Antibody (N39) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB254398100	Novus Biologicals NBP254398100UG	100 μg
NB254398200	Novus Biologicals NBP254398200UG	200 μg

Description

IFN-alpha 2 Monoclonal specifically detects IFN-alpha 2 in Human samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: IFN-alpha 2
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Biologically Active Proteins
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 3440
• Immunogen: Purified recombinant human IFN-alpha2
• Primary or Secondary: Primary
• Content And Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 22 kDa

• Clone: N39
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: alpha-2a interferon, IFNA, IFN-alpha-2, IFN-alphaA, INFA2, interferon alpha 2b, interferon alpha A, interferon alpha-2, Interferon alpha-A, interferon, alpha 2, LeIF A, MGC125764, MGC125765
• Gene Symbols: IFNA2
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.

For Research Use Only