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Invitrogen™ IgD Monoclonal Antibody (IA6-2), eFluor™ 506, eBioscience™, Invitrogen™

Description
Description: This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding. Applications Reported: This IA6-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IA6-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
Specifications
Specifications
| Antigen | Human IgD |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | IA6-2 |
| Concentration | 5 μL/Test |
| Conjugate | eFluor 506 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene | IGHD |
| Gene Accession No. | P01880 |
| Gene Alias | IGHD; immunoglobulin heavy constant delta |
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