IgG F(ab)2 Goat anti-Mouse, DyLight 488, Polyclonal, Seconday Antibody, Novus Biologicals™

Goat Polyclonal Antibody

Supplier:  Novus Biologicals NBP261929G0.5ML

Catalog No. NB008601

Description

IgG F(ab')2 Polyclonal specifically detects IgG F(ab')2 in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.

Specifications

• Antigen: IgG F(ab)2
• Classification: Polyclonal
• Conjugate: DyLight 488
• Formulation: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
• Immunogen: Mouse IgG-F(ab')2 Fragment
• Quantity: 0.5 mL
• Primary or Secondary: Secondary
• Target Species: Mouse
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Dilution: Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 488. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
• Content And Storage: Store at 4°C. Do not freeze.