IgG F(ab)2 Goat anti-Mouse, DyLight 550, Polyclonal, Seconday Antibody, Novus Biologicals™

Catalog No.	Quantity
NB008604	0.5 mL

Catalog No. NB008604 Supplier Novus Biologicals Supplier No. NBP261929R0.5ML

Description

Goat Polyclonal Antibody

IgG F(ab')2 Polyclonal specifically detects IgG F(ab')2 in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.

Specifications

• Antigen: IgG F(ab)2
• Applications: Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: DyLight 550
• Dilution: Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
• Formulation: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
• Host Species: Goat
• Immunogen: Mouse IgG-F(ab')2 Fragment
• Purification Method: Affinity Purified
• Quantity: 0.5 mL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 550. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
• Target Species: Mouse
• Content And Storage: Store at 4°C. Do not freeze.
• Isotype: IgG