IL-1 beta/IL-1F2 Antibody, Biotin, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP142768

Catalog No. NBP142768

Description

IL-1 beta/IL-1F2 Polyclonal specifically detects IL-1 beta/IL-1F2 in Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: IL-1 beta/IL-1F2
• Classification: Polyclonal
• Conjugate: Biotin
• Formulation: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide
• Gene Alias: catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
• Host Species: Rabbit
• Molecular Weight of Antigen: 17 kDa
• Quantity: 0.1 mg
• Research Discipline: Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Gene ID (Entrez): 3553
• Reconstitution: Reconstitute with 100 ul deionized water (or equivalent)
• Content And Storage: Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Concentration: LYOPH
• Dilution: Western Blot 1:2000 - 1:10000, Flow Cytometry 1:10-1:1000, ELISA 1:20000-1:100000, Immunohistochemistry 1:1000-1:5000, Immunohistochemistry-Paraffin 1:10-1:500
• Gene Accession No.: P10749, P10749, P10749, P10749
• Gene Symbols: IL1B
• Immunogen: This IL-1 beta/IL-1F2 Antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta/IL-1F2 produced in E.coli. The MW of recombinant mouse IL-1 beta/IL-1F2 was 17 kDa. (Uniprot: P10749)
• Purification Method: Column Chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody is primarily directed against mature, 17,000 MW mouse IL-1beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1beta or mouse IL-1alpha based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured 31,000 precursor IL-1beta will be recognized by this antibody.
• Target Species: Mouse, Rat
• Form: Purified

For Research Use Only