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IL-1 beta/IL-1F2 Antibody, Biotin, Novus Biologicals™
SDP

Catalog No. NBP142768 Shop All R&D Systems Products
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NBP142768 0.1 mg
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Catalog No. NBP142768 Supplier Novus Biologicals Supplier No. NBP142768
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Description

Rabbit Polyclonal Antibody

IL-1 beta/IL-1F2 Polyclonal specifically detects IL-1 beta/IL-1F2 in Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

Antigen IL-1 beta/IL-1F2
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration LYOPH
Conjugate Biotin
Dilution Western Blot 1:2000 - 1:10000, Flow Cytometry 1:10-1:1000, ELISA 1:20000-1:100000, Immunohistochemistry 1:1000-1:5000, Immunohistochemistry-Paraffin 1:10-1:500
Formulation Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide
Gene Accession No. P10749, P10749, P10749, P10749
Gene Alias catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Gene Symbols IL1B
Host Species Rabbit
Immunogen This IL-1 beta/IL-1F2 Antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta/IL-1F2 produced in E.coli. The MW of recombinant mouse IL-1 beta/IL-1F2 was 17 kDa. (Uniprot: P10749)
Molecular Weight of Antigen 17 kDa
Purification Method Column Chromatography
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Primary or Secondary Primary
Gene ID (Entrez) 3553
Test Specificity This antibody is primarily directed against mature, 17,000 MW mouse IL-1beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1beta or mouse IL-1alpha based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured 31,000 precursor IL-1beta will be recognized by this antibody.
Reconstitution Reconstitute with 100 ul deionized water (or equivalent)
Target Species Mouse, Rat
Content And Storage Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG
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