Invitrogen™ IL-10 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA546912

Catalog No. PIPA546912

Description

In sandwich ELISAs, less than 1% cross-reactivity with recombinant canine IL-10 and recombinant porcine IL-10 is observed and less than 0.4% cross-reactivity with recombinant human IL-10, recombinant mouse IL-10, recombinant rat IL-10, and recombinant feline IL-10 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.15 EU per 1 μg of the antibody by the LAL method.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Antigen: IL-10
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IL10
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
• Host Species: Goat
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100034187
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ELISA, Neutralization, Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q28374
• Gene Symbols: IL10
• Immunogen: E. coli-derived recombinant equine IL-10 Ser19-Asn178.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Horse
• Product Type: Antibody
• Isotype: IgG