DescriptionIL-12/IL-35 p35 Monoclonal antibody specifically detects IL-12/IL-35 p35 in Human samples. It is validated for Intracellular Staining by Flow Cytometry, Immunocytochemistry.
|Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL|
|P29460 (p40) & P29459 (P35)|
|Reconstitute at 0.5 mg/mL in sterile PBS.|
|Detects human IL-12/IL-35 p35 in direct ELISAs. Detects the IL-12/IL-35 p35 subunit either as part of the IL-12 p40/p35 heterodimer or as the IL-12/IL-35 p35 monomer. This antibody does not detect the IL-12/IL-23 p40 subunit.|
|Flow Cytometry, Immunocytochemistry|
|Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.|
|S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p40 and human IL-12 p35 Ile23-Ser328 of p40, Arg23-Ser219 of p35 Accession # P29460 (p40) & P29459 (P35)|
|Protein A or G purified from cell culture supernatant|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.|
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