IL-3 beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-271-8637	Invitrogen™ 500P177GBT500UG	500 μg

Description

AA Sequence of recombinant protein: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC. Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-3beta. Anti-Rat IL-3beta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat IL-3beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-3beta (500-P177G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-3beta. Western Blot: To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P177GBT-1MG will be provided as 2 x 500 μg

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.

Specifications

• Antigen: IL-3 beta
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Goat
• Target Species: Rat
• Gene Accession No.: P04823
• Gene Symbols: Il3
• Primary or Secondary: Primary
• Content And Storage: -20°C

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene ID (Entrez): 24495
• Immunogen: E.coli-derived Recombinant Rat IL-3beta.
• Purification Method: Antigen affinity chromatography
• Gene: Il3