Novus Biologicals™ IL-6 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IL-6.

Specifications

• Assay Range: 3.13 pg/mL
• Assay Sensitivity: 3.13-200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IL-6.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: B cell stimulatory factor-2, B-cell differentiation factor, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFN-beta-2, IL-6B-cell stimulatory factor 2, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6
• Gene ID (Entrez): 3569
• Gene Symbol: IL6
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 27 ng of recombinant Human IL-6.
  Capture Antibody: 1 mg/mL of mouse anti-Human IL-6 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human IL-6 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Biologically Active Proteins, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity, Neuroscience
• Storage Requirements: Storage is content dependent.
• Target: IL-6
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.