Invitrogen™ IL-9 Monoclonal Antibody (RM9A4), eFluor™ 660, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-809-180	Invitrogen™ 50809180	25 μg
50-112-4467	Invitrogen™ 50809182	100 μg

Description

Description: This monoclonal RM9A4 antibody reacts with mouse Interleukin-9 (IL-9). IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta. Applications Reported: This RM9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This RM9A4 antibody has been tested by intracellular staining followed by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empiric...

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Antigen: IL-9
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: eFluor 660
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15247
• Gene ID (Entrez): 16198
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: RM9A4
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Mouse
• Gene: Il9
• Gene Alias: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
• Gene Symbols: Il9
• Product Type: Antibody