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PeproTech IL-9 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P5950UG
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500P5950UG 50 μg
500P59100UG 100 μg
500-P59-1MG 1 mg
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-9. Anti-Murine IL-9-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-9 matrix. Sandwich ELISA: To detect Murine IL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-9 (500-P59Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-9. Western Blot: To detect Murine IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IL-9
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Il9
Gene Accession No. P15247
Gene Alias cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
Gene Symbols Il9
Host Species Rabbit
Immunogen E.coli-derived Recombinant Murine IL-9.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 16198
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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