IL-9 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P5950UG	Invitrogen™ 500P5950UG	50 μg
500P59100UG	Invitrogen™ 500P59100UG	100 μg
500-P59-1MG	Invitrogen™ 500P591MG	1 mg

Description

AA Sequence of recombinant protein: MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-9. Anti-Murine IL-9-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-9 matrix. Sandwich ELISA: To detect Murine IL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-9 (500-P59Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-9. Western Blot: To detect Murine IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Antigen: IL-9
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Mouse
• Gene Accession No.: P15247
• Gene ID (Entrez): 16198
• Immunogen: E.coli-derived Recombinant Murine IL-9.
• Purification Method: Antigen affinity chromatography
• Gene: Il9

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
• Gene Symbols: Il9
• Primary or Secondary: Primary
• Content And Storage: -20°C