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Novus Biologicals™ INSTA-Blot Human Tissues
SDP

Catalog No. NBP230113 Shop All R&D Systems Products
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Catalog No. NBP230113 Supplier Novus Biologicals™ Supplier No. NBP230113
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Description

Applications: Western Blot

  • Screening proteins from various human tissues
  • Contains 11 human tissue lysates (20μg total protein per lane) on 4-20% Tris-Glycine mini gel
  • Prepared from resolution by SDS-PAGE at 125V for 1 hour
  • Proteins are electro-blotted for 2 hours at 25V onto PVDF membrane
  • Stained with amido black for visualization of proteins

Recommended Storage

Store at room temperature

Specifications

For Use With (Application) Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276) .
Surface Western Blot
Quantity 1 Membrane
Material PVDF
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