Novus Biologicals™ INSTA-Blot Human Tissues

Description

Applications: Western Blot

• Screening proteins from various human tissues
• Contains 11 human tissue lysates (20μg total protein per lane) on 4-20% Tris-Glycine mini gel
• Prepared from resolution by SDS-PAGE at 125V for 1 hour
• Proteins are electro-blotted for 2 hours at 25V onto PVDF membrane
• Stained with amido black for visualization of proteins

Recommended Storage

Store at room temperature

Specifications

• For Use With (Application): Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276) .
• Surface: Western Blot
• Quantity: 1 Membrane
• Material: PVDF