Insulin Mouse, Clone: E2-E3 + 2D11-H5 (same as INS04 + INS05), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP2342610.2MG

Catalog No. NBP234261B

Description

Insulin Monoclonal antibody specifically detects Antigen in Bovine, Human, Mouse, Porcine, Rabbit samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting.

Specifications

• Antigen: Insulin
• Clone: E2-E3 + 2D11-H5 (same as INS04 + INS05)
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
• Gene Accession No.: P01308
• Gene Alias: IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
• Host Species: Mouse
• Isotype: IgG
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Storage Requirements: Store at 4C.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Format: Purified
• Gene Symbols: INS
• Immunogen: Full length (1-84 amino acid) purified pig insulin, conjugated to KLH (E2-E3 and 2D11-H5)
• Molecular Weight of Antigen: 6 kDa
• Quantity: 0.2 mg
• Research Discipline: Diabetes Research, Immune System Diseases, Immunology, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 3630
• Target Species: Human, Mouse, Porcine, Bovine, Rabbit