Insulin Mouse, Clone: SPM139, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232974F	Novus Biologicals NBP2329740.02MG	0.02 mg
NBP232974A	Novus Biologicals NBP2329740.1MG	0.1 mg
NBP232974B	Novus Biologicals NBP2329740.2MG	0.2 mg

Description

Insulin Monoclonal specifically detects Insulin in Human, Porcine, Bovine, Mouse (Negative), Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Insulin
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Porcine, Bovine, Mouse (Negative), Rat (Negative)
• Gene Accession No.: P01308
• Gene ID (Entrez): 3630
• Immunogen: Full length (1-84 amino acid) purified pig insulin, conjugated to KLH
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 6 kDa

• Clone: SPM139
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Diabetes Research, Immune System Diseases, Immunology, Stem Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
• Gene Symbols: INS
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.