Integrin alpha 5 beta 1 Antibody (BMC5), Novus Biologicals™

Rat Monoclonal Antibody has been used in 2 publications

Supplier:  Novus Biologicals NBP2297880.025ML

Catalog No. NB019463

Description

Integrin alpha 5 beta 1 Monoclonal specifically detects Integrin alpha 5 beta 1 in Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen, Functional, Immunohistochemistry-Paraffin (Negative).

Specifications

• Antigen: Integrin alpha 5 beta 1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Ascites with No Preservative
• Gene Symbols: ITGA5
• Immunogen: Mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.
• Quantity: 0.025 mL
• Primary or Secondary: Primary
• Test Specificity: Antibody was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes.
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Isotype: IgG2b κ

• Applications: Functional Assay, Immunohistochemistry (Frozen), Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Clone: BMC5
• Dilution: Western Blot, Flow Cytometry 10ul/ml, Immunohistochemistry 1:10-1:500, Immunoprecipitation 2 uL, Immunohistochemistry-Frozen 1:10-1:500, Functional 10ul/ml
• Gene Accession No.: P08648
• Host Species: Rat
• Purification Method: Unpurified
• Regulatory Status: RUO
• Gene ID (Entrez): 3678
• Target Species: Mouse
• Form: Ascites

For Research Use Only