AccuPrime™ GC-Rich DNA Polymerase Non-distribution product as customer accommodation.

High-specificity, high-yield PCR amplification of GC-rich templates

Manufacturer: Invitrogen 12337024

 View all options for this product

Catalog No. 12-337-024

  Check Availability
Add to Cart


    Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
    Gel Compatibility Gel Compatibility: High
    Includes AccuPrime GC-Rich DNA Polymerase is supplied with two 5X reaction buffers and a separate vial of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A is for genomic targets. Reaction buffer B is for use with cDNA, plasmid, or lambda targets.
    Quantity 1000 reactions
    Storage Requirements Store at -20°C.

    AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

    • Yield: high yields for targets up to 5kb in length
    • Specificity: AccuPrime accessory proteins for improved PCR specificity
    • Sensitivity: sensitivity down to 5ng of template DNA
    • Robust and specific amplifications
    • Built-in hot start

    The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

    Unit definition
    One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

    Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit