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Invitrogen™ DNA Polymerase I
Description
Includes
1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5-10 units/μL.
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions
50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.
- Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
- Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Cloning, Restriction Enzyme Cloning
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C). |
| Description | DNA Polymerase |
| Form | Liquid |
| Polymerase | DNA Polymerase I |
| Exonuclease Activity | 3' - 5', 5' - 3' |
| Quantity | 250 U |
| Shipping Condition | Wet Ice |
| Hot Start | No |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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