DNA Polymerase I, Large (Klenow) Fragment Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Manufacturer: Invitrogen 18012021

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Catalog No. 18-012-021

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    Specifications

    Product Type Polymerase (1), DNA
    Includes DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
    For Use With (Application) Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
    Quantity 100 units

    Applications:
    Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

    Source:
    Purified from E. coli expressing the Klenow fragment on a plasmid.

    Performance and Quality Testing:
    SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

    Unit Definition:
    One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template·primer.

    Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

  • Description & Specifications

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