M-MLV Reverse Transcriptase Non-distribution product as customer accommodation.

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

Manufacturer: Invitrogen 28025021

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Catalog No. 28-025-021

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    Concentration 200U/μL
    Includes 1 × 1mL M-MLV RT (200U/μL); 1mL 5X First Strand Buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM Magnesium Chloride]; 500μL 100mM DTT; 200,000 units
    Packaging Type Tube
    Quantity 1000 reactions
    Storage Requirements Store at -20deg.C
    Temperature (English) Operating 98.6deg.F
    Temperature (Metric) Operating 37deg.C
    Test Sensitivity Low

    Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.

    • Thermostability—optimal activity at 37°C
    • Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
    • Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
    • Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
    • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
    • ·Concentration: 200U/µLUnit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
    • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50µL for 10 minutes at 37°C

    • Optimal reaction temperature: 37°C
    • Final product size: 7kb or less

    PCR & Real-Time PCR, Reverse Transcription

  • Description & Specifications

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