Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Catalog Number	Mfr. No.	No. of Reactions
11-754-100	Invitrogen™ 11754100	100 Reactions
11-754-500	Invitrogen™ 11754500	500 Reactions

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™) or LUX™ fluorogenic primers.

• Efficiency: Offers significant time and cost savings for increased throughput
• Convenience: Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity: No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range: Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility: Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
• For use with ABI 7000, 7300, 7700, and 7900(HT) instruments

This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit containing a separate tube of ROX Reference Dye, is also available.

Order Info

Shipping Condition: Dry ice

Specifications

• Concentration: 2X
• Reaction Speed: Fast or Standard
• Product Line: CellsDirect™, Platinum™, SuperScript™
• Sample Type: Cells

• Detection Method: Primer-probe
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• For Use With (Application): RT-PCR

For Research Use Only. Not for use in diagnostic procedures.