Ribonuclease H Non-distribution product as customer accommodation.

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

Manufacturer: Invitrogen 18021071

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Catalog No. 18-021-071

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    Quantity 120 units
    Storage Requirements Store at -20°C in a non-frost-free freezer

    • Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
    • Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
    • Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
    • Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50µL for 20 min. at 37°C

    Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

  • Description & Specifications

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