Ribonuclease H Non-distribution product as customer accommodation.

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

Manufacturer: Invitrogen 18021071

 View all options for this product

Catalog No. 18-021-071

  Check Availability
Add to Cart


    Quantity 120 units
    Storage Requirements Store at -20°C in a non-frost-free freezer

    • Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
    • Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
    • Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
    • Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50µL for 20 min. at 37°C

    Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit