Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit

Description

Includes

Reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50μg total RNA or 0.2-5μg mRNA into double-stranded cDNA.

Requires

Primer

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A⁺-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

• Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
• Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
• Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 μg total RNA or 0.2-5 μg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Labeling Method: Direct Labeling
• Optimal Reaction Temperature: 42°C
• Product Line: SuperScript™
• Product Type: cDNA Synthesis Kit
• Quantity: 50 Reactions
• Reverse Transcriptase: SuperScript™ II
• Shipping Condition: Wet Ice
• For Use With (Application): Microarray Analysis
• Final Product Type: cDNA (Double-Stranded)
• Sample Type: RNA

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.