T7 RNA Polymerase Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

Manufacturer: Invitrogen 18033019

 View more versions of this product

Catalog No. 18-033-019

  Check Availability
Add to Cart

    Specifications

    Product Type Polymerase, T7 RNA
    Includes T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mMmgCl2, 10mM spermidine-(HCl)3, 125mM NaCl], vial of 100mM DTT
    For Use With (Application) Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis
    Quantity 2500 units

    Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

    • Application: Synthesis of labeled and unlabeled RNA transcripts.
    • Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
    • Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
    • Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
    • Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl2, 2mM spermidine-(HCl)3, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1µCi [3H]GTP, 1µg SphI-cut pT7L13, and enzyme in 50µL for 10 min. at 37°C

    Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis

  • Description & Specifications

Related Products


Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit