Invitrogen™ IRAK1 Recombinant Rabbit Monoclonal Antibody (19H32L12)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 700025

Catalog No. PI700025

Description

This antibody is predicted to react with mouse based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

Specifications

• Antigen: IRAK1
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P51617, Q62406
• Gene Symbols: Irak1
• Immunogen: A peptide corresponding to amino acids 579-591 of human IRAK1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Western Blot
• Clone: 19H32L12
• Conjugate: Unconjugated
• Gene: Irak1
• Gene Alias: AA408924; Il1rak; interleukin 1 receptor associated kinase 1; interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; Irak1; IRAK-1; IRAK1-S; mPLK; pelle; Pelle homolog; pelle-like protein kinase; Plpk; RGD1563841
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 16179, 3654
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid