Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152), Novus Biologicals™

Catalog No.	Quantity
NBP24515601	0.1 mg
NBP24515600	0.02 mg
NBP24515602	0.2 mg

Catalog No. NBP24515601 Supplier Novus Biologicals Supplier No. NBP2451560.1MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Isocitrate Dehydrogenase 1/IDH1 Monoclonal specifically detects Isocitrate Dehydrogenase 1/IDH1 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Isocitrate Dehydrogenase 1/IDH1
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Classification: Monoclonal
• Clone: IDH1/1152
• Concentration: 0.2mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Accession No.: O75874
• Gene Alias: Cytosolic NADP-isocitrate dehydrogenase, EC 1.1.1.42, IDCD, IDH, IDP, IDPC, isocitrate dehydrogenase [NADP] cytoplasmic, isocitrate dehydrogenase 1 (NADP+), soluble, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, Oxalosuccinate decarboxylase, PICD
• Gene Symbols: IDH1
• Host Species: Mouse
• Immunogen: Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein
• Molecular Weight of Antigen: 46 kDa
• Purification Method: Protein A or G purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 3417
• Test Specificity: It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
• Target Species: Human
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only