Kappa Light Chain Antibody (KLC709), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2342590.2MG

Catalog No. NBP234259B

Description

Kappa Light Chain Monoclonal antibody specifically detects Antigen in Human samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Simple Western.

Specifications

• Antigen: Kappa Light Chain
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
• Host Species: Mouse
• Molecular Weight of Antigen: 22.5 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, Immunology
• Gene ID (Entrez): 3514
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: KLC709
• Dilution: Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 0.5-1ug/ml
• Gene Accession No.: P01601
• Gene Symbols: IGKC
• Immunogen: Recombinant human Ig kappa chain
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ